ISSN 1308-8734 | E-ISSN 1308-8742
1 Department of Physiology, Çukurova University School of Medicine, Adana, Turkey  
2 Department of Pharmacology, Çukurova University School of Medicine, Adana, Turkey  
Eurasian J Med ; : -

Abstract

Objective: Neuropathic pain (NP) is an inescapable stressor that significantly affects nervous and endocrine system functions. In this study we investigated the effect of neuropathic pain on female reproductive function – using the number of oocytes as an index – and the copulation ratios of female mice, with and without males. We also examined whether these symptoms stopped after injecting the opioid analgesic tramadol.


Materials and Methods
: Tight ligation of the partial sciatic nerve was performed to produce neuropathy, and allodynia was assessed in the cold-plate test. A superovulation protocol was applied to control, sham, neuropathy, and neuropathy + tramadol groups. Each group was divided into two subgroups according to two housing conditions: female alone or female with a male. After induction, oocytes/zygotes were isolated from the ampulla of female mice. Total oocyte number, oocyte maturation, and the copulation ratio were determined.


Results
: The results showed that allodynia, which is a prominent NP symptom was detected in all neuropathic mice and tramadol (50 mg/kg, ip) reversed these effects. The results also showed that NP decreased oocyte the maturation and copulation rates of mice and tramadol reversed all of these effects.


Conclusion
: In conclusion, we suggest that NP affects reproductive performance by altering regulation neuroendocrine mechanisms. Prospective studies that determine levels of cortisol, fertility hormone, cytokine, and other potential endogenous substances in NP animals are needed to clarify the roles in reproduction of these suggested mechanisms.
 


Cite this article as
: Dagilgan S, Erdogan S, Aksu F. Tramadol Reverses the Effects of Neuropathic Pain on Oocyte Maturation and Copulation Ratio in Mice. Eurasian J Med 2018; 50: DOI: 10.5152/eurasianjmed.2017.17199

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