ISSN 1308-8734 | E-ISSN 1308-8742
1 Midwifery Magister Program, Brawijaya University, Malang, East Java and Polytechnic of Health Denpasar, Bali, Indonesia  
2 Department of Obstetrics and Gynecology, Dr. Saiful Anwar Regional Public Hospital, Malang, East Java, Indonesia  
3 Laboratory of Parasitology, Brawijaya University School of Medicine, Malang, East Java, Indonesia  
Eurasian J Med ; : -

Abstract

Objective: The aim of this study was to investigate the effect of N. sativa ethanol extract on the nitric oxide (NO) level and renal arteriol diameter of a preeclampsia mouse model.


Materials and Methods
: This experimental study was conducted using a post-test only control group design. Thirty BALB/c mice were divided into six groups, one negative control (normal pregnant mice), one positive control (preeclampsia model), and four groups of preeclampsia mice treatment with varying doses of N. sativa (500 mg/BW/day, 1000 mg/BW/day, 1500 mg/BW/day, and 2000 mg/BW/day). Ethanol extract of N. sativa was given for five days. Data were analyzed by analysis of variance.


Results
: We detected significant differences in the NO level of the preeclampsia mouse model and those that were given the ethanol extract. The animals given ethanol extract of N. sativa had NO levels of 85.77±4.47 μM (500 mg/BW/day), 189.04±6.01 μM (1000 mg/BW/day), 226.56±2.13 μM (1500 mg/BW/day), and 207.98±4.74 μM (2000 mg/BW/day). The mean diameter of renal arterioles showed significant differences in the treatment group of dose 1000, 1500, and 2000 mg (15.15±2.21b μm, 16.35±2.52b μm, and 15.76±3.03b μm, respectively).


Conclusion
: N. sativa ethanol extract treatment increases NO levels and enlarges the renal arteriol diameter of a preeclampsia mouse model in a dose-dependent manner.


Cite this article as
: Purnamayanti NMD, Windu SC, Poeranto S. Effect of Nigella sativa Ethanol Extract on the Nitric Oxide Content and Renal Arteriole Diameter of a Pre-eclampsia Mouse Model. Eurasian J Med 2018; 50: 10.5152/eurasianjmed.2018.17123

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