ISSN 1308-8734 | E-ISSN 1308-8742
Original Article
Investigation of the Anticancer Mechanism of Isoorientin Isolated from Eremurus Spectabilis Leaves via Cell Cycle Pathways in HT-29 Human Colorectal Adenocarcinoma Cells
1 Department of Physiology, Atatürk University School of Medicine, Erzurum, Turkey  
2 Department of Medical Biology, Pamukkale University School of Medicine, Denizli, Turkey  
3 Department of Pharmaceutical Botany, Atatürk University School of Pharmacy, Erzurum, Turkey  
Eurasian J Med ; : -
DOI: 10.5152/eurasianjmed.2018.17403
Key Words: Isoorientin, colorectal cancer, cell cycle pathway
Abstract

Objective: Isoorientin (ISO) is a flavonoid compound extracted from plant species. The goal of this study was to determine the potential antiproliferative effects of ISO in HT-29 human colorectal adenocarcinoma cell line in vitro, specifically on cell viability, apoptosis, and cell cycle pathways.


Materials and Methods
: The cytotoxic effect of ISO isolated from E. spectabilis was measured using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay in HT-29 cell lines. Total RNA was isolated using Tri-Reagent protocol. The effects of ISO on apoptosis-related gene were detected using real-time polymerase chain reaction (RT-PCR). The findings were analyzed using ΔΔCT method and evaluated using a computer program. Volcano plot analysis was used for comparing groups and the data obtained were statistically analyzed using Student’s t-test.


Results
: According to XTT result analysis, the 50% inhibitory concentration (IC50) value of ISO was 125 μM at the 48th h in HT-29 cells. The RT-PCR analysis in HT-29 cells showed that CCND1, CDK6, casp-3, casp-8, Bax, Bcl-2, CHEK1, CHEK2, and ERCC1 expressions were reduced in ISO-treated cells compared with those in the control group of cells. p53, p21, caspase-9, and ATM- and Rad3-related pathway were increased in the ISO-treated group of cells compared with those in the control group of cells (p<0.05).


Conclusions
: ISO affected the proliferation of colorectal cancer (CRC) cells via cell cycle pathways. It also altered apoptosis gene expression. These results demonstrated that ISO can be a therapeutic agent for CRC treatment; however, more studies are needed to investigate its mechanism of actions.


Cite this article as
: Gundogdu G, Dodurga Y, Elmas L, Yilmaz Tasci S, Karaoglan ES. Investigation of the anticancer mechanism of isoorientin isolated from Eremurus spectabilis leaves via cell cycle pathways in HT-29 human colorectal adenocarcinoma cells. Eurasian J Med 2018; 50: DOI: 10.5152/eurasianjmed.2018.17403.

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