ISSN 1308-8734 | E-ISSN 1308-8742
Original Article
Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema
1 Department of Medical Biochemistry, Eskisehir Osmangazi University School of Medicine, Eskisehir, Turkey  
2 Department of Health Services, Eskisehir Osmangazi University Vocational School of Health Services, Eskisehir, Turkey  
3 Department of Histology and Embryology, Eskisehir Osmangazi University School of Medicine, Eskisehir, Turkey  
4 Department of Medical Services and Techniques, Eskisehir Osmangazi University Vocational School of Health Services, Eskisehir, Turkey  
Eurasian J Med 2019; 51: 42-48
DOI: 10.5152/eurasianjmed.2019.18368
Key Words: Brain injury, conivaptan, ischemia–reperfusion, mannitol, neuronal damage
Abstract

 

Objective: The aim of this study was to compare the effects of conivaptan, an arginine vasopressin antagonist, and mannitol, a sugar alcohol, on cerebral ischemia-induced brain injury and edema in rats.

 

Materials and Methods: Fifty-eight 8-week-old male Sprague Dawley rats were randomly divided into five groups: control, ischemia–reperfusion (I/R)+saline, I/R+mannitol, I/R+10 mg/ml conivaptan, and I/R+20 mg/ml conivaptan. Cerebral ischemia was induced by common carotid artery occlusion for 30 minutes. Saline, mannitol, or conivaptan were administered intravenously at the onset of reperfusion. Blood and brain tissue samples were taken at the 6th hour of reperfusion. The electrolytes (Na+–K+–Cl−), osmolality, arginine vasopressin, albumin, progranulin (PGRN), neuron-specific enolase (NSE), and myeloperoxidase activity were measured in rat serum samples. Brain frontal/hippocampal sections were stained with hematoxylin–eosin and TUNEL techniques to evaluate histopathological changes.

 

Results: Statistical analyses revealed that conivaptan caused significant changes in the electrolyte, NSE, and PGRN levels and osmolality when compared with mannitol. Conivaptan treatment showed positive effects on serum biochemistry and tissue histology.

 

Conclusion: Our findings revealed that conivaptan shows more diuretic activity than mannitol and triggers neither any damages nor edema in the brain tissue. This study may provide beneficial information for the development of treatment strategies for ischemia-related cerebrovascular diseases.

 

Cite this article as: Can B, Oz S, Sahinturk V, Musmul A, Alatas IO. Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema. Eurasian J Med 2019; 51(1): 42-8.

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