ISSN 1308-8734 | E-ISSN 1308-8742
1 Department of Orthopaedics and Traumatology, Cumhuriyet University, Sivas, Turkey  
2 Department of Nutrition and Dietetics, Cumhuriyet University of Health Sciences, Sivas, Turkey  
Eurasian J Med ; : -


Objective: In this study, the aim was to determine the antibiofilm activity of Mentha longifolia essential oil (EO) against biofilms forming on in vitro implant surfaces.


Materials and methods: E. faecalis, E. coli, S. aureus, P. aeruginosa, and K. pneumoniae and C. albicans biofilms were used. Stainless-steel and titanium samples were grouped as control, water diluted, no EO addition and reducing amounts of EO doses. The six microorganisms included in the study were investigated to see if there were differences between the doses on the implant surfaces. The eradication effect of the EO in samples investigated with electron microscope was classified as 0: none, 1: mild, 2: moderate and 3: severe. The chemical composition of the EO was determined with gas chromatography.


Results: There was no difference observed between implant surfaces in terms of biofilm formation. While S. aureus and C. albicans were observed to be most susceptible, the most resistant was identified as P. aeruginosa. The chemical composition of M. longifolia essential oil according to gas chromatography comprised 61.40% carvacrol and 0.23% thymol.


Conclusion: In vitro, M. Longifolia EO was shown to be effective against gram - / + and fungal biofilms forming on the surface of stainless-steel and titanium implants.


You may cite this article as: Pazarcı Ö, Tutar U, Kılınç S. Investigation of the Antibiofilm Effects of Mentha longifolia Essential Oil on Titanium and Stainless-Steel Orthopaedic Implant Surfaces. Eurasian J Med 2019; DOI: 10.5152/eurasianjmed.2019.18432.

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