The Eurasian Journal of Medicine
Original Article

Brain-Derived Neurotrophic Factor Levels in Mesenchymal Stem Cell Culture Fluid Under Different Culture Conditions


Department of Histology and Embryology, Başakşehir Çam and Sakura City Hospital, İstanbul, Turkey


Department of Pediatrics, Kanuni Sultan Süleyman Training and Research Hospital, İstanbul, Turkey


Department of Histology and Embryology, Etlik Zübeyde Hanim Women’s Health Training and Research Hospital, Ankara, Turkey


Department of Histology and Embryology, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, Turkey


Department of Physiology, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, Turkey

Eurasian J Med 2023; 55: 25-31
DOI: 10.5152/eurasianjmed.2021.0138
Read: 739 Downloads: 319 Published: 01 February 2023

Objective: Mesenchymal stem cells are used in various fields, such as cellular therapy, regeneration, or tissue engineering. It has been shown that they exhibit many protective factors and also work as a modulating chief within the region in which they are administered. There are studies on both the therapeutic and neuroprotective effects of brain-derived neurotrophic factor. Also, there are many studies on the improvement of culture conditions for in vitro reproduction of mesenchymal stem cells, which can be obtained from many sources in various bodies, such as adipose tissue and Wharton’s jelly. Improving and standardizing these culture conditions will increase the effectiveness and reliability of stem cell therapies. Studies evaluating many culture conditions, such as O2 level, type of medium, monolayer culture, and the transition from in vitro 3D models, are ongoing.

Materials and Methods: In our study, groups were formed by using stem cells originating from adipose tissue and Wharton’s jelly. Stem cell cultures were made using Hillex-II and Pronectin-F microcarriers. Cell culture O2 level was adjusted as 1% and 5% for each group separately. Enzyme-linked immunosorbent assay was used to analyze brain-derived neurotrophic factor levels in stem cell culture fluid.

Results: The highest brain-derived neurotrophic factor level in mesenchymal stem cells culture medium was observed in an adipose-derived stem cell culture with an in vitro fertilization (non-treated) dish, using a Hillex microcarrier in a 1% O2 microenvironment.

Conclusion: As a result of our observations, we think that cells could exhibit greater therapeutic potential in a dynamic adhesion environment.

Cite this article as: Çiçek G, Çiçek M, Utlu Özen E, Aktan TM, Özdengül F, Duman S. Brain-derived neurotrophic factor levels in mesenchymal stem cell culture fluid under different culture conditions. Eurasian J Med 2023;55(1):25-31.

EISSN 1308-8742