The Eurasian Journal of Medicine
Original Article

Evaluation of Necrotic, Edematous and Inflammatory Changes in Bone Marrow and Soft Tissue After Irrigation with Different Concentrations of NaOCl Solution: An Experimental Study in Rat Tibia

1.

Department of Orthopaedics and Traumatology, Giresun University School of Medicine, Giresun, Turkey

2.

Department of Pathology, Giresun University School of Medicine, Giresun, Turkey

3.

Department of Infectious Diseases and Clinical Microbiology, Ministry of Health Giresun University Prof. Dr. A. Ilhan Ozdemir Training and Research Hospital, Giresun, Turkey

4.

Department of Pathology, Ministry of Health Giresun University Prof. Dr. A. Ilhan Ozdemir Training and Research Hospital, Giresun, Turkey

5.

Department of Biostatistics, Marmara University School of Medicine, Istanbul, Turkey

6.

Department of Pharmacology, Giresun University School of Medicine, Giresun, Turkey

Eurasian J Med 2020; 52: 243-248
DOI: 10.5152/eurasianjmed.2020.19216
Read: 100 Downloads: 78 Published: 26 March 2020

Objective: We aimed to investigate effects of different concentrations (0.5-5%) of Sodium Hypochlorite (NaOCl) solution on healthy tissues, and determine the optimal concentration that does not harm to tissue.

Materials and Methods: 30 tibias of 15 male Wistar albino rats were used. The tibias were randomly divided into 5 groups (Salin, 0.5%, 1%, 2.5%, and 5% NaOCl). Tibias were reamed intramedullary, and irrigated with 20 ml of saline or increasing concentrations of (0.5-5%) NaOCl. The tibias were embedded in paraffin and the sections were stained with hematoxylin-eosin. All sections were assessed for edema, acute inflammation, or necrosis according to their density, in bone marrow and soft tissues.

Results: Pairwise comparisons revealed that irrigation of tibia with saline or 0.5% NaOCl solutions was not statistically significant in terms of necrosis in the bone marrows (p=0.320). However, irrigation of rat tibia with saline caused less necrosis in the bone marrows compared to high concentrations (1%, 2.5%, and 5%) of NaOCl (saline and 1% NaOCl, p=0.017; saline and 2.5% NaOCl, p=0.0007; saline and 5% NaOCl, p=0.001).

Conclusion: As an irrigation solution, the effects of 0.5% NaOCl are similar to those of saline in terms of edema, inflammation, and necrosis. There is a need for evaluation of necrosis for extended periods such as one week or one month by immunohistochemical methods and flow cytometry.

Cite this article as: Aytekin K, Duman A, Aytekin FY, et al. Evaluation of Necrotic, Edematous and Inflammatory Changes in Bone Marrow and Soft Tissue After Irrigation with Different Concentrations of NaOCl Solution: An Experimental Study in Rat Tibia. Eurasian J Med 2020; 52(3): 243-8.

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