The Eurasian Journal of Medicine
Original Article

The Effects of Agomelatine Treatment on Lipopolysaccharide-Induced Septic Lung Injuries in Rats

1.

Clinical Research, Development and Design Application, and Research Center, Atatürk University, Erzurum

2.

Department of Pharmacology, Namık Kemal University Faculty of Medicine, Tekirdağ, Turkey

3.

Department of Pharmacology, Atatürk University Faculty of Medicine, Erzurum, Turkey

4.

Department of Histology and Embryology, Atatürk University Faculty of Medicine, Erzurum, Turkey

Eurasian J Med 2021; 53: 127-131
DOI: 10.5152/eurasianjmed.2021.20342
Read: 70 Downloads: 32 Published: 28 May 2021

Objective: We designed an experimental model of sepsis in rats to investigate the effects of agomelatine (AGO) on lung tissues using molecular and histopathological methods. 

Materials and Methods: In our experimental model, the 32 rats were divided into 4 groups: group 1: control group (HEALTHY); group 2: lipopolysaccharide group (LPS); group 3: LPS plus 50 mg/kg AGO group (LPS + AGO50); and group 4: LPS plus 100 mg/kg AGO group (LPS + AGO100). An LPS-induced sepsis model was performed to replicate the pathology of sepsis. Rats from all 4 groups were killed after 12 hours, and their lungs were quickly collected. To investigate the therapeutic strategy, we evaluated tumor necrosis factor-alpha (TNF-α) and nuclear factor-kappa B (NF-κB) messenger RNA expressions by real-time polymerase chain reaction using molecular methods and lung tissue damage indicators using histopathological methods. 

Results: The expressions of TNF-α and NF-κB were reduced in the groups treated with AGO. The histopathology results supported the molecular results.

Conclusion: In this experimental study, we demonstrated for the first time the positive effects of AGO on LPS-induced sepsis in lung tissue using molecular and histopathological methods, indicating that it contributes to the prevention of lung damage.

Cite this article as: Köse D, Yüksel TN, Halıcı Z, Çadırcı E, Gürbüz MA. The Effects of Agomelatine Treatment on Lipopolysaccharide-Induced Septic Lung Injuries in Rats. Eurasian J Med 2021; 53(2): 127-31.

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