The Eurasian Journal of Medicine
Original Article

7,8-Dihydroxy Flavone Induces Apoptosis via Upregulation of Caspase-3 in Human Hepatocarcinoma Cell


Department of Medical Laboratory Techniques, Selçuk University Vocational School of Health Sciences Medical Services and Techniques Institution, Konya, Turkey


Department of Biochemistry, Selçuk University Faculty of Science, Konya, Turkey

Eurasian J Med 2023; 55: 199-203
DOI: 10.5152/eurasianjmed.2023.22283
Read: 736 Downloads: 321 Published: 25 October 2023

Objective: 7,8-Dihydroxyflavone, a tyrosine kinase receptor agonist, is a flavonoid that has recently gained the attention of researchers due to its anticancer properties. Nevertheless, molecular pathways of 7,8-dihydroxyflavone for hepatocarcinoma are uncertain. Our aim was to identify the impact of 7,8-dihydroxyflavone on human hepatocarcinoma.

Material and Methods: Human hepatocarcinoma cell line-7 cells were used as human hepatocarcinoma cells, and 7,8-dihydroxyflavone was applied to the cells at various doses. The cytotoxic and apoptotic effects of 7,8-dihydroxyflavone were determined with Alamar Blue and flow cytometry. The properties of 7,8-dihydroxyflavone on the mRNA expressions related with Bcl-2, Bax, cleaved-caspase-3 genes, and protein expressions were determined via quantitative real-time polymerase chain reaction and western blot analysis, respectively.

Results: 7,8-Dihydroxyflavone-enhanced cell death in human hepatocarcinoma cell line-7 via the overexpression of cleaved-caspase-3 (P=.003) and decreased Bcl-2 (P=.038) protein levels. Furthermore, cleavedcaspase-3 mRNA overexpression (P=.001) markedly led to 7,8-dihydroxyflavone-induced apoptosis.

Conclusion: 7,8-Dihydroxyflavone could promote apoptotic cell death by modulating caspase pathways and suppressing antiapoptotic protein. These characteristics may mediate to clinical practice of 7,8-dihydroxyflavone for prevention and therapy of hepatocarcinoma.

Cite this article as: Abuşoğlu G, Durmuş Mİ, Karakurt S. 7,8-dihydroxy flavone induces apoptosis via upregulation of caspase-3 in human hepatocarcinoma cell. Eurasian J Med., 2023;55(3):199-203.

EISSN 1308-8742