The Eurasian Journal of Medicine
Original Article

The Role and Antagonistic Effects of miR-16-5p in the Regulation of ADP-Ribosylation Factor-Like Tumor Suppressor Gene 1 in Lung Cancer Cells

1.

Department of Pharmacology, Tekirdağ Namik Kemal University Faculty of Medicine, Tekirdağ, Turkey

2.

Department of Medical Services and Techniques, Adiyaman University Vocational School of Health Sciences, Adiyaman, Turkey

3.

Department of Medical Biology, Adiyaman University Faculty of Medicine, Adiyaman, Turkey

Eurasian J Med 2023; 55: 204-207
DOI: 10.5152/eurasianjmed.2023.23073
Read: 653 Downloads: 261 Published: 25 October 2023

Objective: ADP-ribosylation factor-like tumor suppressor gene 1 is a member of the Ras superfamily of small guanosine triphosphatases that are known to be involved in multiple regulatory pathways in the multistage development of human cancers. Also, ADP-ribosylation factor-like tumor suppressor gene 1 expression levels have been reported to be dramatically lower in both cancer cell lines and tumor tissues compared to controls. Accordingly, defects in the regulation of the ADP-ribosylation factor-like tumor suppressor gene 1 gene seems have key tumor suppressive effects in the formation and development of human cancers including lung cancer. Moreover, microRNAs regulating the expression of ADP-ribosylation factor-like tumor suppressor gene 1 have not been described previously. Accordingly, the present study aimed to reveal the influence of miR-16-5p on the regulation of ADP-ribosylation factor-like tumor suppressor gene 1 gene.

Materials and Methods: A549 lung adenocarcinoma cells were used. For the overexpression and silencing experiments of miR-16-5p synthetic microRNA mimics and inhibitors were used, respectively. Gene expression analyses were achieved with the help of quantitative real-time polymerase chain reaction.

Results: MiR-16-5p was identified to be predictive target of ADP-ribosylation factor-like tumor suppressor gene 1 and directly targets the expression of ADP-ribosylation factor-like tumor suppressor gene 1 as revealed by the overexpression and silencing experiments. Specifically, it was found that miR-16-5p-overexpressed A549 cells showed a decrease in ADP-ribosylation factor-like tumor suppressor gene 1 gene expression, whereas miR16-5p-suppressed cells showed an increase in expression. These findings possibly suggest that miR-16-5p is the direct regulatory microRNA that posttranscriptionally regulates the expression of ADP-ribosylation factor-like tumor suppressor gene 1.

Conclusion: Collectively, miR-16-5p seems to be a key regulatory molecule involved in the posttranscriptional regulation of the ADP-ribosylation factor-like tumor suppressor gene 1, and it might be responsible for the downregulation of this gene in lung cancer.

Cite this article as: Yüksel TN, Bozgeyik E, Bozgeyik İ. The role and antagonistic effects of miR-16-5p in the regulation of ADP-ribosylation factor-like tumor suppressor gene 1 in lung cancer cells. Eurasian J Med., 2023;55(3):204-207.

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EISSN 1308-8742