The Eurasian Journal of Medicine
Original Article

Effect of Citrullus colocynthis Extract on Glycated Hemoglobin Formation (In Vitro)


Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran


Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran


Department of Clinical Biochemistry, Afzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Eurasian J Med 2020; 52: 47-51
DOI: 10.5152/eurasianjmed.2020.19223
Read: 678 Downloads: 277 Published: 27 February 2020

Objective: Diabetes mellitus (DM) is typically a disorder of carbohydrate, fat, and protein metabolism. It develops due to a lack of or loss associated with insulin and/or resistance to insulin. Regarding complications of chemical substance use, drugs with few complications and high-reliability tannins are needed. This study aimed to determine the effect and mechanism of action of Citrullus colocynthis extract on the formation of glycated hemoglobin (HbA1c).

Materials and Methods: A solution containing hemoglobin and glucose was incubated for 1, 2, 3, 4, 30, and 60 days by adding Citrullus colocynthis extract or glutathione. Quantitative measurement of HbA1c was performed using ion-exchange chromatography. Data were analyzed using ANOVA and two-way repeated measures test. A p<0.05 was considered statistically significant.

Results: The Citrullus colocynthis extract in hyperglycemic conditions and with increasing time reduced the formation of HbA1c and thus inhibited the production of glycated proteins. By increasing the time and after initiation of reaction of extract concentrations (0, 0.1, 0.3, 0.5, and 1 g/dL), presently, there was a significant decrease in the formation of HbA1C compared to those in the control group (p<0.05). The decrease in glycation has been dose dependent.

Conclusion: Therefore, Citrullus colocynthis could directly reduce the formation of HbA1c.

Cite this article as: Karimabad MN, Niknia S, Golnabadi MB, Poor SF, Hajizadeh MR, Mahmoodi M. Effect of Citrullus colocynthis Extract on Glycated Hemoglobin Formation (In Vitro). Eurasian J Med 2020; 52(1): 47-51.

EISSN 1308-8742